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首页 > 过刊浏览>2023年第44卷第2期 >370-380. DOI:10.13872/j.1000-0275.2023.0027
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水稻果皮花青素含量的全基因组关联分析
作者:
作者单位:

1.湖南农业大学农学院;2.中国科学院亚热带农业生态研究所

基金项目:

湖南省杰出青年基金项目（2021JJ10041）


Genome-wide association analysis of anthocyanin content in rice seed pericarp
Author:
Affiliation:

1.College of Agronomy, Hunan Agricultural University;2.Institute of Subtropical Agriculture, Chinese Academy of Sciences

Fund Project:

Hunan Outstanding Youth Fund (2021JJ10041)

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    摘要:

    花青素对人类健康具有重要的保健功能，培育富含花青素的功能性水稻品种是未来绿色健康农业发展的必然需求。然而目前与水稻果皮花青素含量相关的基因资源还十分有限，不利于有色稻米品种的种质创新和遗传改良。为了全面发掘水稻果皮花青素的基因资源，本研究结合花青素无损伤检测和全基因组关联分析方法，以533份水稻种质作为供试材料，检测到了13个果皮花青素含量关联QTL位点，这些QTL位点中包含了除Rc、Rd、Rb及OsMYB3已知与花青素相关的基因外，还包括17个候选基因。通过对候选基因同源性及表达模式分析，初步确定8个MYB基因与1个bHLH基因为新的水稻果皮花青素的候选基因。该研究结果首次全面剖析了水稻果皮花青素的遗传基础，为健康功能性水稻品种的选育提供理论基础与基因资源。

    Abstract:

    Anthocyanins in plants play an important role in modulating human health. The cultivation of rice varieties rich in anthocyanins is inevitable to fulfil the demand for developing green and healthy agricultural practices in the future. However, the current genetic resources related to anthocyanin content in rice pericarp are still limited. This is not conducive to germplasm innovation and genetic improvement of colored rice varieties. To comprehensively explore the genetic resources regulating anthocyanin content in rice pericarp, noninvasive detection of anthocyanin was combined with genome-wide association analysis. Using this approach, 13 QTLs associated with anthocyanin content in the pericarp were detected in 533 rice germplasms. These QTLs included 17 candidate genes in addition to Rc, Rd, Rb, and OsMYB3. Through the analysis of homology and expression patterns of these candidate genes, eight MYB and one bHLH gene were preliminarily identified as novel candidate genes associated with anthocyanin content in rice pericarp. This study comprehensively analyzed the genetic basis of anthocyanins in rice pericarp for the first time, providing a theoretical basis and identifying novel genetic resources for the breeding of healthy functional rice varieties.

    参考文献
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何展坤,崔延春,徐庆国,毛东海. 水稻果皮花青素含量的全基因组关联分析[J]. 农业现代化研究, 2023, 44(2): 370-380
HE Zhan-kun, CUI Yan-chun, XU Qing-guo, MAO Dong-hai. Genome-wide association analysis of anthocyanin content in rice seed pericarp[J]. Research of Agricultural Modernization, 2023, 44(2): 370-380

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  • 收稿日期:2023-01-16
  • 最后修改日期:2023-03-21
  • 录用日期:2023-03-21
  • 在线发布日期: 2023-05-06
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