Abstract:With global climate change and the transformation of agricultural production methods, reducing carbon emissions in agriculture, particularly the carbon footprint of rice production, has become essential for promoting sustainable agricultural development. This study aims to clarify the effects of rice varieties and fertilization practices on the yield and carbon footprint of double-cropping rice. Using an integrated approach combining in-situ field monitoring and laboratory analysis, the Life Cycle Assessment (LCA) method was employed to evaluate the comprehensive impacts of different fertilization treatments and rice varieties (T1: 300 kg/hm2 nitrogen fertilizer + Xiangzaoxian45 + Yuzhenxiang; T2: 300 kg/hm2 nitrogen fertilizer + 10 t/hm2 organic fertilizer + Xiangzaoxian45 + Yuzhenxiang; T3: 300 kg/hm2 nitrogen fertilizer + Xiangzaoxian45 + Jingliangyouhuazhan; T4: 300 kg/hm2 nitrogen fertilizer + 10 t/hm2 organic fertilizer + Xiangzaoxian45 + Jingliangyouhuazhan) on carbon emissions, carbon sequestration, and grain yield. The results reveal that agricultural inputs dominate the carbon emissions of double-cropping rice production, contributing 54.54% to 57.47% of the total emissions, with chemical fertilizer and organic fertilizer accounting for 23.88% to 28.63% and 10.00%, respectively. Agricultural operations account for 19.40% to 23.07%, while direct carbon emissions contribute 22.34% to 24.89%. Carbon sequestration by double-cropping rice ranges from 48.29 to 62.59 t/hm2, with T4 increasing carbon sequestration by 29.61%, 15.65%, and 17.10% compared to T1, T2, and T3, respectively. In the late rice season, the Jingliangyouhuazhan variety combined with organic fertilizer contributes the most to carbon sequestration. While the addition of organic fertilizer does not significantly affect grain yield during the early rice season, it significantly enhances dry matter accumulation and grain yield in the late rice season when using the Jingliangyouhuazhan variety. Considering livestock manure management, carbon emissions, carbon sequestration, and grain yield across the cultivation process, the application of organic fertilizer proves effective in reducing the carbon footprint of double-cropping rice. Among the treatments, the Jingliangyouhuazhan variety with organic fertilizer demonstrates the optimal performance.