复合菌剂抑制辣椒疫病的土壤细菌群落特征
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作者单位:

1.湖南农业大学园艺学院;2.中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室

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基金项目:

国家自然科学基金(42201068),湖南省自然科学基金(2023JJ40647),广西八桂学者项目。


Soil bacterial community characteristics in effectively suppressing pepper phytophthora blight by composite microbial agents
Author:
Affiliation:

1.College of Horticulture,Hunan Agricultural University,Changsha;2.Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha

Fund Project:

National Natural Science Foundation of China (42201068); Hunan Provincial Natural Science Foundation (2023JJ40647); Guangxi Bagui Scholarship Program.

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    摘要:

    根际促生菌能通过调控土壤微生物群落组成及功能有效防控作物土传病害。辣椒疫病是由疫霉菌(Phytophthora capsici)引起的严重土传病害之一,然而,目前针对根际促生菌抑制辣椒疫病的效应及其与土壤微生物群落之间关联的研究欠缺。本研究以长期有机培育的健康土壤为材料,通过平板对峙法,筛选并复配出具有显著拮抗病原菌的复合菌剂,通过盆栽实验探究该复合菌剂对辣椒疫病的防治效应及其微生物群落特征。拮抗实验表明,复合菌剂与单一菌株相比能够显著增加对病原菌的拮抗率。盆栽实验表明,接种复合菌剂与对照(接种辣椒疫霉菌)相比,辣椒疫病的发病率和致死率显著降低了14.3%和23.8%,细菌群落α多样性显著下降,细菌群落组成发生改变,与诱导植物系统抗性的细菌群落双组分系统功能显著增加,其中显著增加的波特氏菌属与土壤中病原菌的数量显著负相关。本研究结果为辣椒实际生产中应用复合菌剂防控辣椒疫病提供了技术支撑。

    Abstract:

    Rhizosphere growth promoting microbes can effectively suppress soil-borne diseases by regulating soil microbial community composition and function. Pepper phytophthora blight caused by Phytophthora capsici is one of the detrimental soil-borne diseases of peppers. However, few studies have been conducted to explore the effects of composite microbial agent inoculation on pepper phytophthora blight suppression and soil bacterial community characteristics. The current study aimed to screen composite microbial agents from healthy soil cultivated with long-term application of organic fertilizers, and to assess their effects on suppressing the occurrence and growth of Phytophthora capsici by plate confrontation and pot experiments, with the latter assessing the efficiency of suppressive pepper phytophthora blight and its linkage with bacterial community characteristics. According to the plate confrontation experiment, the composite microbial agents had higher antagonistic effects on Phytophthora capsica growth. According to the pot experiment, the incidence of pepper phytophthora blight was significantly decreased by 14.3%, and the death rate of pepper was significantly decreased by 23.8% under the treatment of composite microbial agent inoculation compared to the control (inoculation with Phytophthora capsici). The α diversity of bacterial community was significantly decreased, the bacterial community composition was significantly altered and the two-component system function of bacterial community was significantly increased under the treatment of composite microbial agent inoculation. The abundance of Bordetella was negatively correlated with the number of Phytophthora capsici. The results provide a technical support for efficiently suppressing pepper phytophthora blight using composite microbial agents in pepper production.

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陈梦,李德军,游甜,葛旭,欧立军,刘秋梅. 复合菌剂抑制辣椒疫病的土壤细菌群落特征[J]. 农业现代化研究, 2024, 45(2): 336-344
CHEN Meng, LI De-jun, YOU Tian, GE Xu, OU Li-jun, LIU Qiu-mei. Soil bacterial community characteristics in effectively suppressing pepper phytophthora blight by composite microbial agents[J]. Research of Agricultural Modernization, 2024, 45(2): 336-344

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  • 收稿日期:2023-12-18
  • 最后修改日期:2024-03-19
  • 录用日期:2024-03-19
  • 在线发布日期: 2024-05-14
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