多年稻草覆盖对土壤荧光假单胞菌phzF功能基因群落组成和多样性的影响
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(1.长江大学农学院,湖北 荆州 434025;2.中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室, 湖南 长沙 410125;中国科学院桃源农业生态试验站,湖南 桃源 415700)

作者简介:

陆晓珍(1988-),女,湖北黄冈人,硕士研究生,主要从事土壤微生物多样性研究;通讯作者:魏文学,研究员,主要从事土壤微生物分子生态研究。

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国家科技支撑计划(编号:2012BAD14B03),国家自然科学基金项目(编号:41271280),湖南省自然科学基金(编号:13JJ3126)。


Effects of Rice Straw Mulching on the Community Structure and Diversity of phzF-containing Pseudomonas fluorescens in Soil
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(1. College of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China;2. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan 410125, China; Taoyuan Station of Agro-ecology Research, the Chinese Academy of Sciences, Taoyuan, Hunan 415700, China)

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    摘要:

    供试土壤采自桃源站红壤旱地稻草覆盖定位试验,利用末端限制性片段多态性(T-RFLP)分子技术研究不同稻草覆盖量对土壤中含phzF基因的荧光假单胞菌种群组成和多样性的影响。结果表明,与化肥处理相比,低量稻草覆盖(LRS)对phzF基因组成的影响较小,而中量和高量稻草覆盖(MRS和HRS)显著改变了phzF基因T-RFs的相对丰度,使含phzF基因的优势荧光假单胞菌种群结构发生明显变化。RDA分析表明,在稻草覆盖条件下土壤速效钾含量是影响phzF基因组成结构的主导因子。每公顷覆盖10000 kg稻草是有利于土壤荧光假单胞菌形成良好结构的稻草用量。研究结果为进一步揭示稻草覆盖对土壤抗病原微生物的影响机理提供了重要的依据。

    Abstract:

    The soil samples were taken from the long-term rice-straw mulching experiment in Taoyuan Agro-ecosystem Research Station. The diversity and community composition structure of soil Pseudomonas fluorescens containing phenazine biosynthesis gene (phzF) were investigated by terminal restriction fragment length polymorphism (T-RFLP) technique. The objective of this study was to understand the responses of phzF-containing P. fluorescens to long-term rice-straw mulching practice. The results showed that low rates of rice straw mulching(LRS) had little influence on the community composition structure of phzF genes, but the middle and high rates (MRS and HRS) resulted in significant changes of the relative abundance of the dominant phzF T-RFs. Redundancy analysis(RDA) indicated that these variations were closely linked to the soil available potassium contents. Among the rice straw mulching treatments, only MRS caused obvious increase of the diversity of phzF-containing P. fluorescens community. It can be suggested that using 10000 kg/hm2 of rice straw would be a suitable amount for habitating the anti-pathogens of P. fluorescens, and as a consequence, soil borne disease might be reduced. The results would be benefit to the understanding the mechanisms of agricultural management on soil anti-pathogenic microorganism.

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陆晓珍,艾天成,张文钊,陈春兰,魏文学. 多年稻草覆盖对土壤荧光假单胞菌phzF功能基因群落组成和多样性的影响[J]. 农业现代化研究, 2014, 35(2): 244-247
LU Xiao-zhen, AI Tian-cheng, ZHANG Wen-zhaO, CHEN Chun-lan, WEI Wen-xue. Effects of Rice Straw Mulching on the Community Structure and Diversity of phzF-containing Pseudomonas fluorescens in Soil[J]. Research of Agricultural Modernization, 2014, 35(2): 244-247

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  • 收稿日期:2013-12-09
  • 最后修改日期:2014-01-19
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  • 在线发布日期: 2014-04-04
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