Abstract: Rapid urbanization has exacerbated the fragmentation of ecological patches. The construction of ecological networks can improve the landscape connectivity between ecological patches, which is of great significance for protecting biodiversity and maintaining ecological service functions. Taking Yuanzhou District, Yichun City, Jiangxi Province as an example, the Morphological Spatial Pattern Analysis (MSPA) method and landscape connectivity assessment were used to identify ecological sources. Considering the impacts of terrain, landscape types and human disturbance, the ecological corridors was simulated by using Least-Cost Path Method and GIS. And then the importance of the ecological corridor was judged by the Gravity Model. Finally, combined with the intersection of the ecological corridor, the hydrological analysis was used to determine the ecological nodes, and ecological network was constructed in order to put forward targeted ecological network optimization suggestions. The results shows that: 1) The ecological source areas are concentrated in the north and south regions of Yuanzhou District, while high comprehensive resistance and low landscape connectivity appeared in the central region. 2) There are 45 ecological corridors in this area, of which 15 are important corridors. These important corridors are mainly distributed in the north regions, particularly No. 1-2 and No. 4-5 important corridors should be protected in priority. 3) Forest and cultivated land are the main components of the ecological corridor. The research results can provide scientific and reasonable reference for Yuanzhou District to coordinate ecological protection and urban development on land use space in the future, and also have certain guiding significance for the construction of ecological networks in other rapidly urbanizing areas.