Abstract: Rural tourism is an important carrier of industrial revitalization as well as a key task of implementing the strategy of rural revitalization. Scientifically measuring and evaluating the carbon footprint and ecological efficiency of rural tourism destination plays a significant role in promoting the prosperity and sustainable development of rural tourism. Based on the perspective of rural revitalization, this paper analyzed the carbon footprint and ecological efficiency of Huangling scenic spot in Wuyuan of Jiangxi Province in 2016 by the process life cycle assessment and ecological efficiency evaluation model. Results show that: 1) the carbon footprint of Huangling scenic spot was 9 732 tons, the per capita carbon footprint was 13.770 kg/person and the average land carbon footprint was 0.649 kg/m2 in 2016; 2) in all aspects of tourism, the carbon footprints of tourism accommodation, tourism transportation, tourism entertainment and shopping accounted for 58.69%, 17.62%, 16.83% and 6.83%, respectively; 3) the ecological efficiency of tourism was 7.85 yuan/kg in Huangling scenic spot in 2016 and there was an obvious difference of ecological efficiency among different links of tourism, the efficiency rates in descending order were transportation, entertainment and shopping, catering and accommodation; and 4) from the perspective of efficiency type, in Huangling scenic spot, the tourism entertainment and shopping belonged to the low carbon and high efficiency type, the tourism transportation belonged to the high carbon and high efficiency type, and the tourism catering and tourism accommodation belonged to the high carbon and low efficiency type. Therefore, to reduce greenhouse gas emissions, this paper suggests: to reduce operation energy consumption, to strengthen low-carbon operation management, and to actively publicize the concept of low-carbon tourism.