Abstract: With warming climate, heat injury strikes rice more frequently. There is few research on heat injury real-time monitor and assessment, which is urgent and valuable in rice production. Thus, based on previous research achievement, this research focused on the monitoring and assessment of heat injury. First, we analyzed satellite remote data to get the daily mean air temperature and maximum air temperature (the cloudy regions’ data was replaced by site temperature data interpolation), which was used to generate the ‘satellite-interpolation’ time series data. Then, based on the laws of vegetation index development, we acquired the data of rice region. After that, we determined if these region’s rice was in the florescence. At last, based on these data, we monitored and assessed the heat injury through the heat injury index, and obtained the injury grade and range. This model can be used to monitor and access the high temperature heat damage on rice at any time point, and can also give the results of the monitoring and evaluation of the overall heat damage during the whole growth period of rice. This model achieved satisfactory results in the experiments in Jiangsu and Anhui and proved the practice value of the model.