Abstract: Small Intestinal Segment Perfusion has been developed to investigate different probiotics products effects on the net absorption of fluid and electrolytes in the small intestine of piglets under bacterial infection. The Ussing chamber provides a physiological system, where microelectrodes are used to detect current changes in intestinal cell membrane ion channels, and in studies of the absorption, permeability and secretion drugs in the intestine. In this paper, we detailed review the invention of Small Intestinal Segment Perfusion and Ussing chamber technology and its application in the pig health breeding research.