Abstract: In purpose to study the effects of in-situ remediation of Cd-contaminated paddy soil by amendments on soil microorganisms, a field experiment that used lime (L), calcium magnesium phosphate (P ), sepiolite (S ) and humic acid powder (H ) as amendments was conducted. And we determined soil microbial biomass (fumigation extraction method), enzyme activity (conventional methods of enzymatic analysis) and soil fungi and bacteria community structure (terminal restriction fragment length polymorphism analysis, T-RFLP). The results indicated that no significant effect of amendments on soil microbial biomass, alkaline phosphatase and urease activities was observed. However, application of lime and sepiolite significantly decreased soil microbial nitrogen (Nmic) by 33.0% and 33.8%, respectively, and the application of calcium magnesium phosphate significantly increased Nmic by 44.9% . And the catalase activity was significantly increased by sepiolite (19.0%). Moreover, significant decrease of soil fungi diversity was observed after sepiolite and humic acid powder applied, and the community structure of bacteria and fungi were changed by the application of amendments. In short, using amendments to remediate Cd- contaminated paddy soil may cause soil microbial ecological risk