文章摘要
基于高通量测序的超级稻不同生育期土壤细菌和古菌群落动态变化
Variations of soil bacterial and archaeal communities during super hybrid rice cultivation based on high throughput sequencing
投稿时间:2017-12-25  修订日期:2018-01-30
DOI:10.13872/j.1000-0275.2018.0010
中文关键词: 超级稻  细菌  古菌  高通量测序  高产区  低产区  生育期
英文关键词: super hybrid rice  bacteria  archaea  high throughput sequencing  high-yield area  low-yield area  growth stage
基金项目:国家自然科学基金项目(31371565)
作者单位E-mail
吴朝晖 湖南杂交水稻研究中心 wuzhaohuihhrrc@163.com 
刘清术 湖南省微生物研究院 volcanoya@126.com 
孙继民 湖南省土壤肥料研究所 sjmy123@163.com 
周建群 湖南省信息与工程研究所 zhoujianqun@126.com 
李鸿波 湖南大学研究生院隆平分院 1214168565@qq.com 
袁隆平 湖南杂交水稻研究中心 yuanlongping@hhrrc.ac.cn 
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中文摘要:
      为阐明超级稻生长不同生育期土壤细菌和古菌群落特征及其影响因素,选取湖南高产区(湖南隆回)和低产区(湖南宁乡)两个水稻种植区,利用Illumina MiSeq高通量测序技术分别对超级稻移栽前,分蘖期、抽穗期、收获期的稻田土壤进行16S rDNA分析,并解析土壤性质对细菌和古菌群落的影响。结果表明:高产生态区土壤微生物多样性在超级稻生育期显著大于移栽前(P<0.05),而低产生态区各时期间差异不显著(P>0.05)。两个生态区的共同优势细菌为Proteobacteria、Acidobacteria、Chloroflexi和Verrucomicrobia,而Bacteroidetes只是高产区的优势细菌类群。Chloroflexi在低产区相对丰度显著大于高产区(P<0.05),Bacteroidetes和Proteobacteria的相对丰度则在高产区显著大于低产区(P<0.05),Acidobacteria和 Verrucomicrobia的相对丰度在两种生产区差异不显著(P>0.05)。低产区古菌数量显著大于高产区(P<0.05),是高产区的2.8~5.5倍。低产区和高产区相对优势古菌群分别是泉古菌门(Crenarchaeota)和广古菌门(Euryarchaeota)。随生育期的变化,Crenarchaeota、Euryarchaeota、Acidobacteria和 Verrucomicrobia的相对丰度呈先减少后增加的趋势,Bacteroidetes和Proteobacteria呈下降趋势,Chloroflexi呈先上升后降低的趋势。RDA分析表明,Proteobacteria的动态变化主要受土壤有机质含量影响,而Bacteroidetes主要受土壤速效磷驱动。高产区和低产区细菌和古菌群落动态变化的主控因子分别是土壤速效氮和速效磷。研究表明,超级稻高产和低产生态区土壤细菌和古菌群落结构差异明显,且随生育期有一定变化,说明土壤速效养分含量是影响土壤细菌和古菌群落的主要因素。
英文摘要:
      The bacterial and archaeal community structure and the influencing factors had been detected in paddy soils of super hybrid rice cultivation areas based on high throughput sequencing. Soil samples were collected from Longhui (HLW, high-yield) and Ningxiang (LNX, low-yield) at four different stages (pre-transplanting, tillering, booting and mature stage) with the cultivation of super hybrid rice “Y Liangyou 900”. Combined with the soil physiochemical properties, the main driving factors were analyzed. Results showed that the bacterial diversity during cultivation was significantly higher than that in pre-transplanting stage in high-yield area (P<0.05), and no significant difference among the four stages in low-yield area (P>0.05). The shared dominant phyla in both areas were Proteobacteria, Acidobacteria, Chloroflexi and Verrucomicrobia, whereas Bacteroidetes just dominated the high-yield area. The relative abundance of Chloroflexi in low-yield area was significantly higher than that in high-yield area (P<0.05), while the relative abundances of Bacteroidetes and Proteobacteria were much higher in high-yield area (P<0.05), and no significant differences of the relative abundances of Acidobacteria and Verrucomicrobia were detected between the two areas (P>0.05). The relative abundance of archaeal community in low-yield area was 2.8-5.5 times higher than that in high-yield area (P<0.05). The dominant phylum was Crenarchaeota in low-yield area, whereas Euryarchaeota dominated the high-yield area. The variations of relative abundances of Crenarchaeota, Euryarchaeota, Acidobacteria and Verrucomicrobia had the same trend with decreasing first and then increasing during the four stages, while the Chloroflexi had an opposite trend. The relative abundances of Bacteroidetes and Proteobacteria were declined through the four stages. RDA analysis indicated that soil organic matter was the most important environmental factor affecting Proteobacteria, while available P was the key factor influencing Bacteroidetes and Proteobacteria communities. The key driving factor in shaping microbial communities in high-yield and low-yield areas was available N and available P, respectively. In a word, the soil bacterial and archaeal communities was obviously different between high-yield and low-yield areas, and varied with the super hybrid rice growth stages. The results revealed that contents of available nutrients were the main factors affecting soil bacterial and archaeal communities.
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